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KMID : 0900920010250030219
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Korean journal of Animal Reproduction
2001 Volume.25 No. 3 p.219 ~ p.225
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Establishment of Embryonic Stem Cells Derived from Rabbit EmbryosRabbit;ES cells;MEF;REF;LIF
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Kang Hyee-Sung
Lim Kyung-Sun
Choi Hwa-Sik
Shin Young-Soo
Jin Dong-Il
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Abstract
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To establish rabbit Embryonic Stem (ES) cells, rabbit one-cell embryos were collected and cultured in vitro to blastocysts. Blastocysts were co-cultured with mouse embryonic fibroblasts (MEF), rabbit embryonic fibroblasts (REF) or 570 cells expressing LIF (SNL). Although rabbit ES cells were isolated with low efficiencies, total 8 ES cell lines were kept in vitro with normal colony shape. The MEF was the best feeder for rabbit ES cell isolation in regard to growth rate and undifferentiated morphology. The doubling time of rabbit ES cells in MEF was about 84 hours and the undifferentiated morphology was maintained following passing and freezing processes. These rabbit ES cells were differentiated into embryoid body following the culture in the uncoated dishes, indicating that they were undifferentiated stem cells.
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KEYWORD
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Rabbit, ES cells, MEF, REF, LIF
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